rabbit polyclonal ire1  (Elabscience Biotechnology)

Journal: Cancer Diagnosis & Prognosis

Article Title: Immunohistochemical Expression of Endoplasmic Reticulum Stress Markers and their Association With Clinicopathological Characteristics and Survival Outcomes in Endometrial Cancer

doi: 10.21873/cdp.10531

Figure Lengend Snippet: Inositol-requiring enzyme 1 (IRE1) immunohistochemical stain: (a) Magnification 100× intensity score 0 (no color reaction), (b) Magnification 100× intensity score 1 (low intensity of color reaction), (c) Magnification 100× intensity score 2 (average intensity of color reaction), and (d) Magnification 100× intensity score 3 (intense color reaction).

Article Snippet: For the detection of IRE1 and PERK expression, tissue sections were incubated with specific primary antibodies: rabbit polyclonal IRE1 (E-AB-93217; Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B-5: sc-377400; Santa Cruz Biotechnology, Dallas, TX, USA), both at a dilution of 1:50.

Techniques: Immunohistochemical staining, Staining

Journal: Cancer Diagnosis & Prognosis

Article Title: Immunohistochemical Expression of Endoplasmic Reticulum Stress Markers and their Association With Clinicopathological Characteristics and Survival Outcomes in Endometrial Cancer

doi: 10.21873/cdp.10531

Figure Lengend Snippet: Clinicopathologic characteristics of endometrial cancer survivors and their association with IRE1-IRS and PERK-IRS expression levels. Statistical comparisons were performed to evaluate potential correlations between biomarker expression and clinical variables.

Article Snippet: For the detection of IRE1 and PERK expression, tissue sections were incubated with specific primary antibodies: rabbit polyclonal IRE1 (E-AB-93217; Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B-5: sc-377400; Santa Cruz Biotechnology, Dallas, TX, USA), both at a dilution of 1:50.

Techniques: Expressing, Biomarker Discovery

Journal: Cancer Diagnosis & Prognosis

Article Title: Immunohistochemical Expression of Endoplasmic Reticulum Stress Markers and their Association With Clinicopathological Characteristics and Survival Outcomes in Endometrial Cancer

doi: 10.21873/cdp.10531

Figure Lengend Snippet: Kaplan-Meier curves for binary IRE1 with 95% confidence intervals for overall (left) and disease-free (right) survival. Crosses denote censoring events. p-Values were calculated using log-rank tests.

Article Snippet: For the detection of IRE1 and PERK expression, tissue sections were incubated with specific primary antibodies: rabbit polyclonal IRE1 (E-AB-93217; Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B-5: sc-377400; Santa Cruz Biotechnology, Dallas, TX, USA), both at a dilution of 1:50.

Techniques:

Journal: Veterinary Research

Article Title: Outer membrane vesicles secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing endoplasmic reticulum stress-mediated autophagy flux blockade

doi: 10.1186/s13567-025-01679-6

Figure Lengend Snippet: APEC OMVs activate UPR signaling to induce ERS . A HD11 cells were treated with 50–200 µg/mL OMVs for 6 h, and the relative mRNA levels of UPR downstream genes ATF4 , CHOP , EDEM1 , ERdj4 , and ATF6 were detected by qPCR. ( n = 3) B HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, and qPCR was used to detect the relative mRNA levels of UPR downstream genes ATF4 , CHOP , EDEM1 , ERdj4 , and ATF6 . ( n = 3) C , D HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, western blotting was used to detect the expression of PERK, p-PERK, eIF2α, p-eIF2α, CHOP, IRE1, p-IRE1, and ATF6 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( D ). ( n = 3). E , F HD11 cells were treated with OMVs (100 µg/mL) for 6 h in the absence or presence of 4-PBA (2 mM), western blot analysis was used to detect the expression of PERK, p-PERK, eIF2α, p-eIF2α, CHOP, IRE1, p-IRE1, and ATF6 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( F ). ( n = 3); n represents three biological replicates; data points indicate independent culture systems. Bar charts display mean ± standard error of the mean (SEM). Two-way ANOVA with Sidak correction was performed (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Rabbit anti-p-IRE1 polyclonal antibody , Abmart, Shanghai, China , 1:5000.

Techniques: Western Blot, Expressing, Software

Journal: Veterinary Research

Article Title: Outer membrane vesicles secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing endoplasmic reticulum stress-mediated autophagy flux blockade

doi: 10.1186/s13567-025-01679-6

Figure Lengend Snippet: OMVs secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing ERS-mediated autophagy flux blockade . APEC-secreted OMVs, upon uptake by HD11 cells, induce ROS accumulation and Ca 2+ release, triggering ERS and activating UPR pathways, including the PERK, IRE1, and ATF6 signaling branches, leading to the expression of stress-related factors such as GRP78/BiP and CHOP. The sustained activation of ERS inhibits autophagosome degradation and disrupts the acidic environment of lysosomes, thereby preventing autophagosomes from fusing with lysosomes and impairing the phagocytic clearance capacity of macrophages. Collectively, these abnormal conditions facilitate APEC survival within HD11 cells and enable immune evasion, ultimately promoting bacterial dissemination and systemic infection in the host.

Article Snippet: Rabbit anti-p-IRE1 polyclonal antibody , Abmart, Shanghai, China , 1:5000.

Techniques: Infection, Expressing, Activation Assay

Journal: Veterinary Research

Article Title: Outer membrane vesicles secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing endoplasmic reticulum stress-mediated autophagy flux blockade

doi: 10.1186/s13567-025-01679-6

Figure Lengend Snippet: APEC OMVs activate UPR signaling to induce ERS . A HD11 cells were treated with 50–200 µg/mL OMVs for 6 h, and the relative mRNA levels of UPR downstream genes ATF4 , CHOP , EDEM1 , ERdj4 , and ATF6 were detected by qPCR. ( n = 3) B HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, and qPCR was used to detect the relative mRNA levels of UPR downstream genes ATF4 , CHOP , EDEM1 , ERdj4 , and ATF6 . ( n = 3) C , D HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, western blotting was used to detect the expression of PERK, p-PERK, eIF2α, p-eIF2α, CHOP, IRE1, p-IRE1, and ATF6 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( D ). ( n = 3). E , F HD11 cells were treated with OMVs (100 µg/mL) for 6 h in the absence or presence of 4-PBA (2 mM), western blot analysis was used to detect the expression of PERK, p-PERK, eIF2α, p-eIF2α, CHOP, IRE1, p-IRE1, and ATF6 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( F ). ( n = 3); n represents three biological replicates; data points indicate independent culture systems. Bar charts display mean ± standard error of the mean (SEM). Two-way ANOVA with Sidak correction was performed (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Rabbit anti-IRE1 polyclonal antibody , Abmart, Shanghai, China , 1:5000.

Techniques: Western Blot, Expressing, Software

Journal: Veterinary Research

Article Title: Outer membrane vesicles secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing endoplasmic reticulum stress-mediated autophagy flux blockade

doi: 10.1186/s13567-025-01679-6

Figure Lengend Snippet: OMVs secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing ERS-mediated autophagy flux blockade . APEC-secreted OMVs, upon uptake by HD11 cells, induce ROS accumulation and Ca 2+ release, triggering ERS and activating UPR pathways, including the PERK, IRE1, and ATF6 signaling branches, leading to the expression of stress-related factors such as GRP78/BiP and CHOP. The sustained activation of ERS inhibits autophagosome degradation and disrupts the acidic environment of lysosomes, thereby preventing autophagosomes from fusing with lysosomes and impairing the phagocytic clearance capacity of macrophages. Collectively, these abnormal conditions facilitate APEC survival within HD11 cells and enable immune evasion, ultimately promoting bacterial dissemination and systemic infection in the host.

Article Snippet: Rabbit anti-IRE1 polyclonal antibody , Abmart, Shanghai, China , 1:5000.

Techniques: Infection, Expressing, Activation Assay

Journal: Frontiers in Physiology

Article Title: Inositol-requiring enzyme 1 alpha is essential for dentinogenesis

doi: 10.3389/fphys.2025.1722417

Figure Lengend Snippet: Immunohistochemistry staining of phosphorylated IRE1α (pIRE1α) protein in the mandibular first molars. (A) Shown are the representative images of immunohistochemistry staining of pIRE1α protein (signal in brown) in the mandibular first molars of 3-week-old Dspp +/+ , Dspp P19L/+ , and Dspp P19L/P19L mice. Each image in (A) is from the middle region of the crown of a sagittally-sectioned mandibular first molar. (A1-A3) are the higher magnification views of the roof-forming odontoblasts (box1), dental pulp cells (box 2) and floor-forming odontoblasts (box 3) in the corresponding images in (A) , respectively. rd, roof dentin; fd, floor dentin; rod, roof-forming odontoblasts; fod, floor-forming odontoblasts; Scale bars: 50 μm in A; 20 μm in (A1-A3). Three independent experiments for IHC staining of pIRE1α show similar results.

Article Snippet: The primary antibodies used in this study included: 1) rabbit anti-phosphorylated IRE1α polyclonal antibody (1:400, Novus Biologicals, NB100-2323); 2) rabbit anti-XBP1 polyclonal antibody that recognizes both unspliced XBP1 (XBP1U) and spliced XBP1 (XBP1S) (1:200, Abcam, ab37152); 3) rabbit anti-XBP1S monoclonal antibody (E9V3E) that specifically recognizes XBP1S ( ; ) (1:50, Cell Signaling Technology, Danvers, MA); 4) rabbit anti-DSPP polyclonal antibody that recognizes both DSP and full-length DSPP (1:2000) ( ); and 5) rabbit anti-DMP1 polyclonal antibody (1:800, #857) ( ).

Techniques: Immunohistochemistry, Staining